Journal: Neuropharmacology

Article Title: Activation of neural stem cells from quiescence drives reactive hippocampal neurogenesis after alcohol dependence

doi: 10.1016/j.neuropharm.2018.01.032

Figure Lengend Snippet: NeuroD1+ immunoreactivity is increased following 14, but not 7, days of abstinence in ethanol-treated rats. Representative photomicrographs of control (A,C) and ethanol-exposed animals (B,D) at 7 days (A,B) and 14 days (C,D) post ethanol exposure. The number of NeuroD1+ cells in the subgranular zone (SGZ) of the hippocampal dentate gyrus was determined using unbiased stereology (E) for ethanol (n=8–9) and controls (n=6–8) and shown as total estimated NeuroD1+ cells ± SEM. GCL, granule cell layer; scale bar = 100 µm; inset scale bar = 20 µm; *p<0.001.

Article Snippet: For anti-NeuroD1, anti-Ki67, and anti-phosphoHistoneH3 an additional antigen retrieval step in either 1× standard sodium citrate or Citra buffer (Biogenex, Fremont, CA) for 1 h at 65°C was required.

Techniques:

Journal: Neuropharmacology

Article Title: Activation of neural stem cells from quiescence drives reactive hippocampal neurogenesis after alcohol dependence

doi: 10.1016/j.neuropharm.2018.01.032

Figure Lengend Snippet: Adult neurogenesis is a multi-step process that begins with Type 1 stem-like radial glial cells that proliferate and divide asymmetrically to generate a progenitor cell (Type 2a) which can then proliferate and/or differentiate into a Type 2b NPC (circular arrows indicate that the cell is able to proliferate (Kempermann et al., 2004). Type 2b NPCs can proliferate and/or differentiate into a Type 3 NPC, which can then proliferate and differentiate into an immature neuron. Fluorescent quadruple immunohistochemistry was designed to identify each of the various progenitor cell subtypes as described in A. For example, NeuroD1 is expressed in Type 2b and Type 3 NPCs and is considered to be a Type 2b cell when it co-expresses with Sox2, but a Type 3 cell when Sox2 is no longer expressed. (B) Representative confocal images are shown: type 1 cell is positive for Ki67, GFAP, and Sox2 but is negative for NeuroD1 (row 1). Type 2a proliferating cells are positive for Ki67 and Sox2 but negative for GFAP and NeuroD1 (row 2). A combination of Ki67+, Sox2+, and NeuroD1+ yet GFAP- signifies a type 2b cell (row 3). Finally, a cell that is only Ki67+ and NeuroD1+ is a type 3 cell (row 4). ML, molecular layer; GCL, granule cell layer; SGZ, subgranular zone; scale bar = 20µm.

Article Snippet: For anti-NeuroD1, anti-Ki67, and anti-phosphoHistoneH3 an additional antigen retrieval step in either 1× standard sodium citrate or Citra buffer (Biogenex, Fremont, CA) for 1 h at 65°C was required.

Techniques: Immunohistochemistry

Journal: BMC Neuroscience

Article Title: Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum

doi: 10.1186/1471-2202-13-117

Figure Lengend Snippet: Radial organization and immunocytochemical patterns at the end of DS1. A . Ectopic expression of pShhiEGFP in an electroporated OT (ED4.5); patches of positive NE cell bodies at the ventricular zone (VZ) and of postmitotic neurons at the premigratory zone (PMZ) can be observed. B . Hematoxylin-Eosin staining. Arrowheads point to mNE cells located along ventricular zone; they show PH3 nuclear labeling ( C ) and Notch reactive cytoplasm ( D ). Arrows point to neurons in the premigratory zone; they display nuclear NeuroD reactivity ( E ) and βIIITub reactive perikarya ( F ). Bar: 10 μm.

Article Snippet: Rabbit polyclonal anti-neurod (NeuroD1) , Lifespan Biosciences , , , Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH Human NeuroD1 (aa 76–91) , 1:100.

Techniques: Expressing, Staining, Labeling

Journal: BMC Neuroscience

Article Title: Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum

doi: 10.1186/1471-2202-13-117

Figure Lengend Snippet: Primary antibodies characteristics

Article Snippet: Rabbit polyclonal anti-neurod (NeuroD1) , Lifespan Biosciences , , , Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH Human NeuroD1 (aa 76–91) , 1:100.

Techniques: Translocation Assay, Recombinant